Fig. 6.

Protein levels of Nox1, GTP-Rac1 and Total-Rac1. BMMs cells were pretreated with various concentrations of polyphyllin VII (PP7) for 2 h. The cells were then induced to differentiate into osteoclasts with or without RANKL and M-CSF. After 48 h, protein levels of Nox1, GTP-Rac1, and Total-Rac1 were assessed by immunoblotting as described in the methods. Panel a Representative image of immunoblotting assay of Nox1, GTP-Rac1 and Total-Rac1 protein. Panel b, c and d: Effect of polyphyllin VII (PP7) on the expression of Nox1 protein (b), GTP-Rac1 protein (c), and Total-Rac1 protein (d). Vertical axes: protein level; horizontal axes: polyphyllin VII (PP7) concentrations (μM). * P < 0.05 as compared to the cells treated with 0 μM of polyphyllin VII