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. 2020 Feb 19;20:57. doi: 10.1186/s12935-020-1119-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — The expression of hsa_circ_0003159 is decreased in GC. a The expression of hsa_circ_0003159 was detected in GC tissues and normal tissues (n = 55) by qRT-PCR. b, c Overall survival (b) and disease-free survival (c) of patients were analyzed according to the expression of hsa_circ_0003159. HR hazards ratio, CI confidence interval. d The level of hsa_circ_0003159 was detected in GC cell lines (NUGC-3, AGS, HS-746T and N87) and normal GES-1 cells by qRT-PCR. e The expression levels of hsa_circ_0003159 and CACNA2D1 were detected in NUGC-3 and AGS cells after treatment of RNase R or not by qRT-PCR. f The abundance of hsa_circ_0003159 was examined in NUGC-3 and AGS cells by qRT-PCR, with U6 and 18S rRNA as internal controls for nuclear and cytoplasm, respectively. *P < 0.05