Fig. 9.

Suppression of neural activity reduces nuclear invaginations in cultured Lrrk2^−/− SPNs. a Co-staining of Lamin B and CTIP2 in the Lrrk2^+/+ SPN cultures treated with vehicle or bicuculline. Scale bar, 10 μm. Arrow marks the site of nuclear invagination. b Percentage of the SPN nuclei containing ≥1 invagination was calculated from three independent Lrrk2^+/+ cultures treated with vehicle or bicuculline. N = 200 neurons per treatment. Unpaired t-test, ^***p = 0.0035. c Co-staining of Lamin B and CTIP2 in the Lrrk2^−/− SPN cultures treated with vehicle or TTX. Scale bar, 10 μm. Arrow marks the site of nuclear invagination. d Percentage of SPN nuclei containing ≥1 invagination was calculated from three independent Lrrk2^−/− cultures treated with vehicle, bicuculline and TTX. N = 200, 200, and 150 neurons for vehicle, bicuculline, and TTX treatment, respectively. 1way ANOVA, ^****p < 0.0001. e Co-staining of Lamin B and CTIP2 in the Lrrk2^RC/RC SPN cultures treated with vehicle or TTX. Scale bar, 10 μm. f Percentage of SPN nuclei containing ≥1 invagination was calculated from three independent Lrrk2^RC/RC cultures treated with vehicle and TTX. N = 100 neurons per treatment, respectively. Unpaired t-test, ^**p = 0.0017