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Pitfalls of human epidermal growth factor receptor 2 immunohistochemistry digital imaging analysis. (a). Air bubbles (red arrow); (b) pigments (red arrow); (c) ductal carcinoma in situ components (red arrow); invasive carcinoma was pointed with black arrow; (d) ink (red arrow); (e) out of focus; (f) rare tumor cells (red arrow). The human epidermal growth factor receptor 2 connectivity was labeled with green lines
