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. 2017 May 12;12(5):442–455. doi: 10.1016/j.ajps.2017.04.001

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© 2017 Shenyang Pharmaceutical University. Production and hosting by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6 — Immunohistochemical analysis of TNF-α in the liver of control and experimental groups of animals (40Χ). Plate's a–f corresponds to the liver sections of groups 1–6, respectively, (A) Control group. (B) DEN-induced group. (C) hesperetin alone treated group. (D) DEN + hesperetin treatment group. (E) Au-mPEG_(5000)-S-HP NPs alone treated group. (F) DEN + Au-mPEG_(5000)-S-HP NPs treated group. (G) Quantitative analysis of TNF-α expression. The number of stained cells/100 cells was counted across ten fields for each slide. Results are expressed as mean ± SD for six rats in each group. Statistical significance at P < 0.05 compared with ^agroup 1, ^bgroup 2 and ^cgroup 4. Arrow represents TNF-α positive stained cells.