Western blot analysis of TNF-α, NF-kB and β-Actin in the liver of control and experimental groups of animals. (A) Lane 1 – Control, Lane 2 – DEN induced group, Lane 3 – hesperetin alone treated group, Lane 4 – DEN + hesperetin treatment group, Lane 5 – Au-mPEG(5000)-S-HP NPs alone treated group, Lane 6 – DEN + Au-mPEG(5000)-S-HP NPs treated groups (B) Densitometric analysis of TNF-α, NF-kB and β-Actin in control and experimental groups of animals. Results are expressed as mean ± SD for six rats in each group. Statistical significance at P < 0.05 compared with agroup 1, bgroup 2 and cgroup 4.