Fig. 2.

The level of anti-PR8 specific IgG1 and IgG2a (A); and the ratio of IgG2a/IgG1 antibody titers (B) of BALB/c mice immunized by In and Im routes, 10d after the last booster injection with different formulations. The assays were performed using an ELISA method in triplicate at different dilutions of serum samples. Significant differences between the IgG1 titers of ALG-coated NPs (In) and none-coated ones was marked as *(P < 0.05) and ***(P < 0.001). Significant difference of IgG2a titers between ALG-coated NPs (In) and none-coated ones was labeled with ###(P < 0.001). Data represented as mean ± SD (n = 6).