Fig. 2.

The characterization of covalent-albumin corona on SP@BSA NPs by SDS-PAGE, taking PLGA@BSA NPs as control. After each centrifugation and resuspension, the supernatant (S) was loaded onto the SDS-PAGE. S1 was diluted 10 times with additional amount of water to avoid overloading the gel. SDS was used to remove the hard adsorption BSA from the NP surface. Meanwhile, the residual NPs were also loaded onto gel to identify the covalent- conjugated BSA.