Figure 3:

Effect of UCKL-1 over-expression on apoptosis induction in the 3T3 mouse fibroblast cell line. Staurosporine (12.5 nM) was added to control and UCKL-1 transfected 3T3 cells to induce apoptosis. Data are a summary of six replicate experiments. (a) Cell counts 22 hours after transfection were normalized to the level of untreated control transfected cells (pControl) (*p = 0.02). (b) UCKL-1 over-expression protected 3T3 cells from staurosporine-induced apoptosis. Caspase activity was measured 22 hours after nucleofection. Activity was normalized to untreated pControl-transfected cells, which was set to 100 (*p = 0.01).