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. Author manuscript; available in PMC: 2020 Feb 20.
Published in final edited form as: Int J Immunol Immunother. 2016 May 24;3(1):10.23937/2378-3672/1410018. doi: 10.23937/2378-3672/1410018

Figure 7:

Figure 7:

Effect of UCKL-1 levels on NF-κB activity in K562 cells. NF-κB activity was measured by transfection of an NF-κB luciferase expression plasmid. (a) Significantly higher levels of NF-κB activity were observed in UCKL-1 transfected than in control transfected cells (*p = 0.02). Significantly less NF-κB activity was present in siUCKL-1-treated cells compared to sicontrol-treated cells (**p = 0.04). Data are a summary of four replicate experiments. (b & c) UCKL-1 increases NF-κB activity in a dose-dependent manner. K562 cells were nucleofected with the NF-κB luciferase reporter plasmid along with 2.5, 5 or 10 μg pFlag-UCKL-1 or 10 μg pControl; (b) NF-κB activity and c UCKL-1 protein levels, were analyzed 24 hours later. There is a dose-dependent increase in NF-κB activity with increasing levels of UCKL-1 (*p < 0.01). Data are a summary of five replicate experiments.