Figure 9:

Effect of UCKL-1 over-expression on IκB levels in the cytoplasm. Cytoplasmic extracts from pControl or pFlag-UCKL-1 transfected K562 cells were analyzed for levels of IκB 20 hours after nucleofection by immunoblotting. Extracts from K562 cells stimulated with TNF-α (5 ng/ml) for 30 minutes were run as a positive control. (a) A representative blot is shown; (b) Quantization of IκB illustrates that there is significantly less IκB in the cytoplasm of UCKL-1 transfected cells than in control-transfected cells. Data are a summary of four experiments (*p ≤ 0.02).