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. 2020 Feb 18;3(3):e201900547. doi: 10.26508/lsa.201900547

Figure 3. DDX11 interacts with Pol δ independently of its FeS cluster.

Figure 3.

(A) Gene Ontology term enrichment analysis of interaction partners obtained upon pull-down of YFP-DDX11 from HeLa Flp-In T-REx cells. (B) Flag pull-down of over-expressed Flag-tagged DDX11 from 293T cells and co-immunoprecipitated endogenous proteins. (C) Reciprocal co-immunoprecipitations of Flag-tagged POLD1 and untagged DDX11, and Flag-tagged DDX11 and untagged POLD1, respectively, extracted from 293T cells. (D) Co-immunoprecipitations of Flag-tagged DDX11 variants and untagged POLD1 from 293T cells. See also Fig S3, Tables S1, and S2. WB, Western blot.