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. 2020 Feb 18;3(3):e201900547. doi: 10.26508/lsa.201900547

Figure 4. DDX11 can remove obstacles from the DNA template ahead of Pol δ.

Figure 4.

(A) Schematic of primer extension assay. Blue ellipse depicts 5′-FAM label on the primer that gets extended by Pol δ. Light grey circle depicts 3′-biotin on the DNA block that was added to prevent primer extension. Numbers indicate lengths of primers and gaps in nucleotides. (B) Time-resolved primer extension assay with substrate as depicted in A (15 nt-long DNA block) or without a DNA obstacle (no block). S denotes substrate only. In all other lanes, 2.5 nM of Pol δ was added in the absence or presence (25 nM) of DDX11 variants. N+7 denotes extension of primer by seven nucleotides. (C) Quantification of primer extension beyond the DNA block in the absence or presence of 25 nM DDX11 variants. Data points indicate values from two independent experiments. Lines connect the theoretical mean values.