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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Exp Eye Res. 2019 Sep 11;188:107798. doi: 10.1016/j.exer.2019.107798

Figure 4. Evaluation of anti-VEGF alone or in combination with PEDF peptides.

Figure 4.

Mice were IVT- injected with anti-mouse VEGF164 (1 μL of 25 μg/mL alone or in combination with PEDF peptides (1 μL 4 mM peptide in the antibody solution) on day −2. The vehicle was Avastin-vehicle per package insert (trehalose, phosphate, NaCl), and was used for preparing all solutions. Animals were sacrificed on day 14 and RPE/choroidal flatmounts were prepared and stained with anti-ICAM-2 as described under “Methods”.The lesion areas were determined using imageJ. Please note a significant inhibition of neovascularization with antibody alone (*P< 0.05) and similar response with antibody and PEDF-335 (*P< 0.05). Statistical significance was enhanced by PEDF-336 (**P< 0.01).