Fig. 5.

Inhibition of CaMKII phosphorylation downregulates CCL5-induced MMP2 expression. (A, B) Cells pretreated with KN (10–50 µM) followed by CCL5 treatment showed inhibition of cleaved MMP2 expression. (C) Glioma cells transfected with CaMKII siRNA followed by CCL5 (10 ng/mL) treatment showed inhibited CaMKII phosphorylation and cleaved MMP2 protein expression. (D) Invasive activity was inhibited in the glioma cells with si-CaMKII followed by CCL5 (10 ng/mL) treatment. (E) The invading cells in (D) were quantified and presented as percent of invading cells. Bar: 100 µm. The data are presented as means ± SE. *P < 0.05, #P < 0.01. Blots are quantified as fold of control.