Fig. 6.

ALT-803 induces pSTAT5 and pERK in NK cells. Healthy donor CD56⁺ NK cells were treated overnight with ALT-803, cetuximab, combination of ALT-803 with cetuximab, or vehicle control and stained for pERK and pSTAT5 via intracellular flow cytometry. Percentage of CD56⁺ NK cells positive for a pSTAT5 and quantified for b fold change in expression and c delta mean MFI (pERK-isotype control expression). Percentage of CD56⁺ NK cells positive for d pSTAT5 quantified for e fold change in expression and f delta mean MFI (pSTAT5-isotype control expression). Means + STD of 3 donors. *P < 0.05 compared to PBS/vehicle control-treated cells