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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Leuk Lymphoma. 2019 Oct 8;61(2):409–419. doi: 10.1080/10428194.2019.1672055

Figure 1.

Figure 1.

Conditioned medium from Jurkat-PTHrP cells had greater bone resorption and calcium release in ex vivo experiments. (A–D) Neonatal calvarial discs co-cultured with conditioned media from Jurkat cells and TRAP-stained for osteoclast activity. Vertical pairs were from the same mouse, thus each PTHrP-pcDNA and MIP-1-pcDNA comparison was internally controlled. (E) Jurkat-PTHrP conditioned medium significantly decreased bone area of calvaria due to increased osteoclastic bone resorption comparedto Jurkat-pcDNA as indicated by a decrease in calvarial area (**p < .01). (F) Co-cultures resulted in higher concentrationsof calcium in the medium containing the calvaria and conditioned medium from the Jurkat-PTHrP cells when compared to Jurkat-MIP-1α and Jurkat-pcDNA cells (*p < .03).