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Published in final edited form as: Adv Exp Med Biol. 2011;705:465–492. doi: 10.1007/978-1-4419-7877-6_25

Fig. 3 — Alignment of deduced amino acid sequences of four β6GlcNAc transferases from human (Homo sapiens) (h), chimpanzee (Pan troglodytes) (c), rat (Rattus norvegicus) (r), mouse (Mus musculus) (m), bovine (Bos taurus) (b), bovine herpesvirus (bhv), dog (Canis familiaris) (dog), and zebra fish (Danio rerio) (d). Multiple sequence alignment was performed with Invitrogen Vector NTI 10 software. The amino acid sequences of dog C2GnT-M and C2GnT-T, and mC2GnT-T used for alignment, include only those that are comparable to those of other β6GlcNAc transferases. The N-terminal amino acid sequences that are excluded are 96 amino acid residues of dog C2GnT-M, 137 residues of dog C2GnT-T, and 133 residues of mC2GnT-T. The N-glycosylation sites (N) are highlighted dark, and the nine cysteines conserved among all β6GlcNAc transferases, the four cysteines conserved among C2GnT-Ms, and the one cysteine conserved among C2GnT-Ts are highlighted light. The potential N-glycosyltaion sites in each sequence are indicated by arrow heads. The accession numbers for these proteins are C2GnT-2/M: bovine, NP_991378.1; bovine herpesvirus, Q80RC7; dog, XP_544703; chimpanzee, XP_510451.2; human, NP_004742.1; mouse, NP_082363.2; rat, NP_775434.1. C2GnT-3/T: chimpanzee, XP_517702.2; human, NP_057675.1; dog, XP_546063.2; mouse, XP_980153.1; zebra fish, NP_963877.1. IGnT: human, Q06430–1; mouse, NP_032131. C2GnT-1/L: bovine, NP_803476.1; mouse, NP_034395.1; rat, NP_071612.1; chimpanzee, XP_001145936.1; human, NP_001091103.1; dog, XP_541274.2. The accession numbers of the corresponding cDNAs are C2GnT-2/M: bovine, NM_205809.1; bovine herpesvirus, NC_002665; dog, XM_544703; chimpanzee, XM_510451.2; human, NM_004751.1; mouse, NM_028087.2; rat, NM_173312.1. C2GnT-3/T: human, NM_016591.1; chimpanzee, XM_517702.2; dog, XM_546063.2; mouse, XM_975059.1; zebra fish, NM_201583.1. IGnT: human, Z19550; mouse, NM_008105. C2GnT-1/L: bovine, NM_177510.2; mouse, NM_010265.2; rat, NM_022276.1; chimpanzee, XM_001145936.1; human, NM_001097634.1; dog, XM_541274.2

Fig. 3 — Alignment of deduced amino acid sequences of four β6GlcNAc transferases from human (Homo sapiens) (h), chimpanzee (Pan troglodytes) (c), rat (Rattus norvegicus) (r), mouse (Mus musculus) (m), bovine (Bos taurus) (b), bovine herpesvirus (bhv), dog (Canis familiaris) (dog), and zebra fish (Danio rerio) (d). Multiple sequence alignment was performed with Invitrogen Vector NTI 10 software. The amino acid sequences of dog C2GnT-M and C2GnT-T, and mC2GnT-T used for alignment, include only those that are comparable to those of other β6GlcNAc transferases. The N-terminal amino acid sequences that are excluded are 96 amino acid residues of dog C2GnT-M, 137 residues of dog C2GnT-T, and 133 residues of mC2GnT-T. The N-glycosylation sites (N) are highlighted dark, and the nine cysteines conserved among all β6GlcNAc transferases, the four cysteines conserved among C2GnT-Ms, and the one cysteine conserved among C2GnT-Ts are highlighted light. The potential N-glycosyltaion sites in each sequence are indicated by arrow heads. The accession numbers for these proteins are C2GnT-2/M: bovine, NP_991378.1; bovine herpesvirus, Q80RC7; dog, XP_544703; chimpanzee, XP_510451.2; human, NP_004742.1; mouse, NP_082363.2; rat, NP_775434.1. C2GnT-3/T: chimpanzee, XP_517702.2; human, NP_057675.1; dog, XP_546063.2; mouse, XP_980153.1; zebra fish, NP_963877.1. IGnT: human, Q06430–1; mouse, NP_032131. C2GnT-1/L: bovine, NP_803476.1; mouse, NP_034395.1; rat, NP_071612.1; chimpanzee, XP_001145936.1; human, NP_001091103.1; dog, XP_541274.2. The accession numbers of the corresponding cDNAs are C2GnT-2/M: bovine, NM_205809.1; bovine herpesvirus, NC_002665; dog, XM_544703; chimpanzee, XM_510451.2; human, NM_004751.1; mouse, NM_028087.2; rat, NM_173312.1. C2GnT-3/T: human, NM_016591.1; chimpanzee, XM_517702.2; dog, XM_546063.2; mouse, XM_975059.1; zebra fish, NM_201583.1. IGnT: human, Z19550; mouse, NM_008105. C2GnT-1/L: bovine, NM_177510.2; mouse, NM_010265.2; rat, NM_022276.1; chimpanzee, XM_001145936.1; human, NM_001097634.1; dog, XM_541274.2