SAHA alone significantly increased the expression of caspase 7 in all four breast cancer cell lines. (A) qRT-PCR using caspase 7 primers was performed for MCF-7 cells after 3-day treatments with the indicated compounds (n=3). GAPDH was used for comparison; (B-D) As above, in MDA-MB-157, MDA-MB-231, and HCC1806 cells (n=3). EGCG alone significantly reduced the expression of caspase 7 in MDA-MB-231 cells (C). Error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines; * p<0.05, ** p<0.01, *** p<0.001.