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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: J Clin Virol. 2019 Sep 16;120:44–50. doi: 10.1016/j.jcv.2019.08.011

Figure 2.

Figure 2.

Probit regression analysis of singleplex versus multiplex CII-ArboViroPlex rRT-PCR. Ten replicates of 10,000, 5,000, 1,000, 500, 250, 100, 60, 50, 40, 20, 10 and 5 of in vitro transcribed viral RNA copies were run in CII-ArboViroPlex assays in singleplex format (one template per assay; red symbols) or in multiplex format (all four viral templates per assay; blue symbols). Probit analysis was performed by MATLAB v12 and trendline curves were calculated in Microsoft Excel v16.16.12 for CHIKV (A), DENV (B), ZIKV (C) and WNV (D). LoDs at 95% CI and p-values (MATLAB) were calculated comparing singleplex and multiplex results. Curves are shown for the range of 5 to 100 copies/Rn; for 250 to 10,000 copies/Rn all replicates (100%) were positive with comparable Ct values in all cases.