Table 2.
Determination of the presumptive and final limit of detection (LOD) of the ZIKV, DENV 1, DENV 2, DENV 3, DENV 4, CHIKV and WNV for the CII-ArboViroPlex rRT-PCR assay with serum specimens and ZIKV with urine specimens.
| ZIKV | DENV 1 | DENV 2 | DENV 3 | DENV 4 | CHIKV | WNV | |
|---|---|---|---|---|---|---|---|
| LoD | |||||||
| Virus concn (Copies/reaction) | 2.58E+02 | 2.19E+01 | 9.35E+00 | 2.32E+02 | 1.44E+03 | 2.70E+02 | 1.05E+01 |
| Dilution factor from stock | 1.00E−05 | 1.00E−05 | 1.00E−05 | 1.00E−05 | 1.00E−05 | 1.00E−05 | 1.00E−06 |
| Confirmation of the LoD | |||||||
| Average Ct (20 replicates) | 31.81 | 32.87 | 32.43 | 31.01 | 31.2 | 31.52 | 33.51 |
| standard deviation | 0.76 | 0.8 | 0.93 | 0.81 | 0.52 | 0.28 | 0.48 |
| Call rate | 20/20 | 20/20 | 20/20 | 20/20 | 20/20 | 20/20 | 20/20 |
| Call rate % | 100% | 100% | 100% | 100% | 100% | 100% | 100% |