Skip to main content
. 2020 Feb 20;15(2):e0226736. doi: 10.1371/journal.pone.0226736

Fig 1. No significant change of Wolbachia density in hosts with hybrid genetic profiles.

Fig 1

The expression of wsp gene was measured by real-time quantitative PCR in NJ♀×TKtet♂, TK♀×NJtet♂, NJtet, TKtet, NJ and TK virgin females at 2 days post-eclosion. wsp gene expression data were normalized with RPS6. The primer sequences are shown in Table 1. The bars indicate standard error. 2−ΔΔCT method was used to calculate the expression level. Significance was determined based on comparison of the ΔCT using Student’s t-tests.