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. 2020 Feb 7;16(2):e1008269. doi: 10.1371/journal.ppat.1008269

Fig 2. Knockdown of host RNA decay factors does not rescue Pol II occupancy.

Fig 2

(A) Representative western blot showing the efficiency of Dis3L2 and Xrn1 knockdown (kd) in MC57G cells, with Gapdh serving as a loading control. Densitometry of band intensity normalized to Gapdh and MC57G mock infection are indicated below blots. (B) ChIP-qPCR of Pol II occupancy at the Rplp0 and Fus promoters with standard deviation. Pol II ChIP was performed on mock and MHV68 WT infected MC57G cells treated with the indicated siRNAs. IgG is from the siControl MHV68 infection condition. (* p < 0.05, ** p < 0.001, *** p < 0.0001 students paired t-test on raw % input values).