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. 2020 Feb 7;14(2):e0008059. doi: 10.1371/journal.pntd.0008059

Fig 2. Trypanosoma ingestion increases A. coluzzii bacterial flora abundance at day 2 and day 5 post-feeding.

Fig 2

The graphs in A and B show median fold change of the total bacteria load in the mosquito midgut using 16S rDNA detection by qPCR and the ribosomal protein rps7 gene as the internal calibrator. qPCR detection was performed at day 2 (D2) and day 5 (D5) post-blood meal. The graph in A shows the effect on the mosquito microbiome from females fed on the mouse infection system. The graph in B shows the effect on the mosquito microbiome from females fed on cultured trypanosomes mixed with sheep blood. -Tryp = mosquitoes fed on blood without trypanosomes; +Tryp = mosquitoes fed on blood containing trypanosomes. The dotted line represents the level of 16S rDNA from -Tryp mosquitoes. The ratio of the normalized 16S rDNA detection in “+Tryp” versus “-Tryp” was calculated using triplicates from the same cDNA dilution. Error bars show median absolute deviation computed by permutation from 3 experiments. *: statistically significant p-value (p<0.05) related to the deltaCt distribution between “+Tryp” and “-Tryp”.