Fig 8. Fitness analysis of Ddn mutants.
(a) M. tuberculosis Ddn mutants are not attenuated in mice. Mean lung colony-forming unit (CFU) counts of the H37Rv strain and isogenic Ddn mutants are shown following low-dose aerosol infection. Error bars represent SEM biological triplicate. Competition of the Δddn mutant compared to isogenic wild type mc2 6230. (b) Growth and survival of the Δddn mutant compared to isogenic wild type in aerobic conditions. (c) Growth and survival of the Δddn mutant compared to isogenic wild type in hypoxic conditions. (A and B) Arrows indicate samples taken for competition assay. (d) Percentage of the Δddn mutant when grown in competition with the isogenic wild type. Two-way ANOVA with Tukey multiple comparisons test where *, p<0.05; **, p<0.01. Red * is comparison between aerobic (Day 7) and Hypoxic (Day 37) at given timepoint, Black * is comparison between aerobic (Day 7) and Hypoxic (Day 70) at given timepoint. Error bars for b, c and d representative of standard deviation biological triplicate.