Fig 6. C9C5 immunoreactivity in the mouse hypothalamus across life.

(A-F) Immunostaining with the C9C5 antibody on coronal sections of the hypothalamus at P4 (A) (n = 3), P10 (B) (n = 3), P20 (C) (n = 4), and at two (D) (n = 4), nine (E) (n = 3) and twelve (F) (n = 4) months. C9C5⁺ cells are detected in hypothalamic nuclei at P10 until adulthood but not at P4. An age-associated decrease of C9C5⁺ cells in the parenchyma but not in the median eminence (ME) was observed (D-F). Magnifications of white boxes show C9C5⁺ cells with a stellate morphology, which were absent at P4 (A) and in twelve month-old animals (F). C9C5⁺ cells are present in the ME at P4 (A). (G) Quantification of C9C5⁺ cells across life (n = 3–4 per age). Means ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. Nuclei stained with DAPI. 3V, third ventricle; mo, months.