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. Author manuscript; available in PMC: 2020 Feb 20.
Published in final edited form as: Nat Protoc. 2016 Aug 4;11(9):1586–1598. doi: 10.1038/nprot.2007.148

Figure 4.

Figure 4

Example log2 ratio profile of RNA pol II binding in Drosophila neural stem cells. DNA was isolated from 50 Drosophila melanogaster larvae for both Dam-polII and Dam-only samples; larvae were torn in half and the anterior halves (which contain the brain) retained for DNA isolation and processing. Expression of the TaDa proteins was driven using a neural stem cell-specific driver (worniu-GAL4) in the presence of tub-GAL80ts and induced for 16 hrs at 29°C from 72 hrs after larval hatching. The gene miranda (mira) illustrated here is required for the correct segregation of cell fate determinants during the division of Drosophila neural stem cells.