FIGURE 2.

RAB7 overexpression upregulates CSR in vivo. (A) Flow cytometry analysis of surface IgD and IgG3 expression in B cells from the spleen in mice before NP-CGG/alum immunization (n=3, mean and s.e.m. in the right panel). (B) Flow cytometry analysis of spleen B cell proliferation (left) and differentiation into plasma cells (middle) and germinal center B cells (right) in mice immunized with NP-CGG in alum for 14 d (n=3, mean and s.e.m.). (C) Flow cytometry analysis of B cell differentiation into germinal center B cells and plasma cells (left), and RAB7 expression (right) in immunized mice, as in (B). Representative of three independent experiments. (D) IgG1 and IgG2a expression in different mouse spleen B cell subsets, as in (C; mean and s.e.m., n=3 in the histogram, right). (E) Surface expression of IgG1 and intracellular expression of RAB7 in germinal center B cells and plasmablasts in immunized mice, as in (C). Representative of three independent experiments. (F) Surface expression of NP-binding BCR in B cells (left; numbers indicate the proportions of NP-specific B cells) and IgG1 expression within NP-specific B cells (right; numbers indicate the proportions of IgG1⁺ cells) in immunized mice, as in (B). Representative of three independent experiments. (G) Levels of different transcripts, as indicated, in B cells from immunized mice, as in (B). Data were normalized to the values in wildtype mice (mean and s.d., triplicates). *, p < 0.05; **, p < 0.01.