FIGURE 3.
Levels of circulating anti-nuclear antibodies were reduced in AID deficient BXSB mice. (A) Sera ANA intensity detection of pan-Ig and IgM autoantibodies in 12-week-old BXSB (n=15) and BXSB.Aicda−/− (n=11) mice. (B) Sera anti-dsDNA autoantibody detection for pan-Ig and IgM isotypes in these same mice. Anti-dsDNA plasma cell ELISPOT analysis of bone marrow (C) and spleen (D) from 12-week-old BXSB (n=5) and BXSB.Aicda−/− (n=6) mice. Spot numbers in C and E are normalized to control wells. Data are presented as mean±SEM. Statistical significance was determined by Mann-Whitney U test. ***p<0.001.