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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Immunol. 2020 Jan 27;204(5):1091–1100. doi: 10.4049/jimmunol.1900501

FIGURE 5.

FIGURE 5.

No effect on plasma cell generation both in vivo and in vitro in the absence of AID. (A) The percentage of plasma cells (CD138+) in either viable splenocytes or viable bone marrow cells were compared between 12-week-old BXSB (n=9) and BXSB.Aicda−/− (n=6) males. (B-D) Enriched B lymphocytes from 8-week-old BXSB (n=3) and BXSB.Aicda−/− (n=3) males were stimulated to differentiate into plasma cells either with anti-CD40 for T cell-dependent (TD) response or LPS for T cell-independent (TI) response. Representative plasma cell staining in viable cells and Annexin V staining in CD138+ cells is shown in B and data are summarized in C and D. Early and late apoptosis were differentiated by Annexin V and 7-AAD respectively. Early apoptosis (early, Annexin V+7-AAD-), late apoptosis (late, Annexin V+7-AAD+) and all apoptosis (apoptosis, Annexin V+) were compared in D. Data are presented as mean±SEM. Statistical significance was determined by Mann-Whitney U test. Results are representative for at least 3 independent experiments.