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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Immunol. 2020 Jan 17;204(5):1334–1344. doi: 10.4049/jimmunol.1900650

Figure 4. IL1 eRNAs regulate transcription.

Figure 4.

MonoMac6 cells were transfected with the indicated 20-bp phosphorothioate antisense oligonucleotides (ASO) designed to knock down eRNAs (two different ASOs each) or an ASO to GFP for 3 hours and stimulated with or without 1μg/ml LPS for 60 minutes. Total RNA was isolated and qRT-PCR was performed to measure levels of eRNAs and mRNAs. A) Knockdown of 327 eRNA significantly decreased the LPS-induction of IL1A but had less effect on IL1B and no effect on IL1RN. B) Knockdown of 299 eRNA significantly decreased the LPS-induction of IL1A and IL1B mRNAs but did not affect the IL1RN transcript levels. Fold change indicates levels compared to the GFP ASO in the unstimulated cells. (n =3, * p<0.05). PCRs were normalized to Actin.