Fig. 6.

Effects of Na_V1.8 on diastolic Ca²⁺ waves. Data are presented as mean ± SEM. P values were calculated using one-way ANOVA with Tukey's test for multiple comparisons or Fisher's exact test (for c, g). a Confocal line scan images of human atrial cardiomyocytes loaded with the Ca²⁺ indicator Fluo-4 representing the occurrence of major diastolic arrhythmogenic Ca²⁺ release events. b Mean frequency of Ca²⁺ waves in control (n = 108 cardiomyocytes/13 patients) and after treatment with A-803467 (n = 87/9) or PF-01247324 (n = 104/11). c Percentage of cells developing arrhythmic events (Ca²⁺ waves or spontaneous transients; n = 24 of 116 cardiomyocytes/13 patients) and effects of Na_V1.8 blockade with A-803467 (n = 7 of 90/9 patients) or PF-01247324 (n = 8 of 104/11 patients). d Representative original line scans of murine atrial cardiomyocytes from SCN10A^−/− and (e) WT mice. f Mean values of Ca²⁺ wave frequency and (g) proportion of cells showing arrhythmic events in SCN10A^−/− (n = 67 cells/7 mice) and WT (n = 80 cells/7 mice) mice and effects of Na_V1.8 inhibition by PF-01247324 (SCN10A^−/−: n = 67 cells/7 mice and WT: n = 70/7) and TTX (SCN10A^−/−: n = 68 cells/7 mice and WT: n = 67/7) in both genotypes