Table 2.
Charging levels of mt tRNAAla constructs (related to Fig. 3).
| mt tRNAAla variant | tRNA charged [µM] | tRNA charged relative to WT [%] |
|---|---|---|
| H. sapiens WT | 2.71 ± 0.03 | 100 ± 1.2 |
| U62C | 2.63 ± 0.03 | 97 ± 1.3 |
| U68C | 2.32 ± 0.08 | 86 ± 3.3 |
| U62C/U68C | 2.74 ± 0.05 | 101 ± 1.9 |
| C6U | 1.55 ± 0.05 | 57 ± 3.0 |
| C6U + U62C | 2.66 ± 0.08 | 98 ± 3.2 |
| C6U + U68C | 2.82 ± 0.07 | 104 ± 2.5 |
| U7C | 1.13 ± 0.05 | 42 ± 4.1 |
| U7C + A66G | 2.94 ± 0.07 | 109 ± 2.5 |
| U7C + U62C | 2.03 ± 0.08 | 75 ± 4.0 |
| U7C + U62C/U68C | 3.05 ± 0.08 | 113 ± 2.6 |
| C28U | 1.26 ± 0.02 | 46 ± 1.7 |
| C28U + G42A | 3.01 ± 0.11 | 111 ± 3.5 |
| C28U + U62C/U68C | 2.86 ± 0.12 | 106 ± 4.3 |
| A31G | 1.31 ± 0.05 | 48 ± 4.2 |
| U39C | 1.08 ± 0.02 | 40 ± 2.2 |
| A31G + U39C | 0.93 ± 0.03 | 34 ± 3.2 |
| A31G + U62C/U68C | 1.62 ± 0.07 | 60 ± 4.3 |
| T. obscurus | 3.19 ± 0.12 | 118 ± 3.7 |
| C69U | 1.41 ± 0.05 | 52 ± 3.5 |
| C69U + U62C | 2.53 ± 0.11 | 94 ± 4.3 |
| C69U + U68C | 2.93 ± 0.13 | 108 ± 4.4 |
| C69U + U62C/U68C | 2.83 ± 0.06 | 105 ± 2.1 |
| A1G/U72C | 1.07 ± 0.03 | 40 ± 3.2 |
| A1G/U72C/U62C/U68C | 2.55 ± 0.07 | 94 ± 2.8 |
| C6U/C28U/A31G/C50U | 0.97 ± 0.01 | 36 ± 0.9 |
| P. coquereli | 3.11 ± 0.07 | 115 ± 2.3 |
Charging level of wild-type (WT) Hs mt tRNAAla is set to 100%. The data represent the mean of two to six independent experiments ± standard error.
T. obscurus: Corresponds to Hs mt tRNAAla with mutations A31G + G26A/G27A/G54A/G57A/G59A/U62C/C65U.
P. coquereli: Corresponds to Hs mt tRNAAla with mutations C6U/C28U/A31G/C50U + A1G/G5A/U19A/G26A/G27A/U39C/G42A/A53G/G58A/U61C.
Source data are provided as a Source Data file.