Table 3.
Kinetics of aminoacylation of tRNAAsp variants by Hs mt AlaRS (related to Fig. 5).
| kcat | Km | kcat/Km | Rel. kcat/Km | Rel. kcat/Km | |
|---|---|---|---|---|---|
| [10−3 s−1] | [µM] | [10−3 s−1 µM−1] | (tRNAAsp)a | (tRNAAla)b | |
| mt Ala(WT) | 9.9 ± 0.6 | 0.46 ± 0.14 | 21.39 ± 6.50 | 1 | |
| mt Asp(WT) | 0.3 ± 0.1 | 11.7 ± 5.4 | 0.03 ± 0.01 | 1 | |
| mt Asp(mtAla-Vc) | 0.8 ± 0.2 | 7.7 ± 4.6 | 0.10 ± 0.06 | 4 | 0.005 |
| mt Asp(mtAla-SLc) | 0.1 ± 0.02 | 1.8 ± 1.1 | 0.07 ± 0.04 | 3 | 0.003 |
| mt Asp(mtAla-SLVc) | 6.7 ± 0.2 | 0.7 ± 0.1 | 9.39 ± 1.50 | 355 | 0.44 |
| mt Asp(proto-1) | 3.0 ± 0.1 | 0.7 ± 0.1 | 4.18 ± 0.58 | 158 | 0.20 |
| mt Asp(proto-2) | 3.6 ± 0.2 | 0.8 ± 0.2 | 4.28 ± 0.84 | 162 | 0.20 |
aActivity relative to wild-type mt tRNAAsp.
bActivity relative to wild-type mt tRNAAla.
cS: Stems; L: (D- & T) Loops; V: V-loop. Means and standard errors were calculated from two to six independent experiments. Source data are provided as a Source Data file.