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. 2020 Feb 20;11:973. doi: 10.1038/s41467-020-14794-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Aleuria aurantia lectin (AAL-555) binding before and after incubation with recombinant HsFUT8 at two different concentrations (650 nM and 3.5 μM) on a selection of glycan structures. b AAL-555-binding profile on a full glycan array before treatment with recombinant HsFUT8. c AAL-555-binding assay after treatment with recombinant HsFUT8 at 650 nM enzyme concentration and d AAL-555-binding assay after treatment with recombinant HsFUT8 at 3.25 μM enzyme concentration. Histogram bars show the average fluorescence RFU (relative fluorescence units) values for four replicate spots on the array. Error bars represent the standard deviation of the average RFU values on the same microarray. N-glycans that show binding towards AAL after incubation with HsFUT8 are highlighted in blue.