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. 2020 Feb 20;11:970. doi: 10.1038/s41467-020-14729-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Quantification of MMA levels by LC-MS/MS; n = 8 mmut^+/+ and n = 12 mmut^del11/del11 zebrafish larvae. b, c Representative images and quantification of the mitochondrial shape (expressed as circularity) in (b) livers and in (c) kidneys of 10-dpf mmut zebrafish (n ≥ 9 and n ≥ 10 randomly selected and non-overlapping fields of views for zebrafish livers and kidneys, respectively). The whole-field images are pooled from three distinct zebrafish kidneys and livers, respectively. Dotted yellow squares contain images at higher magnification. d Oxygen consumption rate (OCR) and individual parameters for basal respiration in 10-dpf-mmut zebrafish, n = 6 mmut^+/+ and 12 mmut^del11/del11 zebrafish larvae. e Zebrafish expressing mito-Grx1-roGFP2 in the liver were outcrossed with mmut^+/del11 zebrafish. Representative images and quantification of the ratio between 405 (blue) and 488 (green) fluorescence intensities, with each point representing the average blue/green fluorescence intensity ratio in a zebrafish liver; n = 11 mmut^+/+ and n = 12 mmut^del11/del11 zebrafish larvae. f Tracking analyses of motor behaviour in 10-dpf-mmut zebrafish fed with a high- or low-protein diet (HP or LP, respectively). Quantification of the distance, with each point representing the average distance covered by an individual zebrafish; n = 28 HP-fed mmut^+/+, n = 26 LP-fed mmut^+/+, n = 23 HP-fed mmut^del11/del11 and n = 27 LP-fed mmut^del11/del11 zebrafish larvae. g Distribution of mmut zebrafish (expressed as the percentage of the total zebrafish larvae) in 5-dpf or in 14-dpf zebrafish fed with either HP or LP diet or in mmut zebrafish stably expressing mmut in the liver; n ≥ 3 biologically independent experiments, with each containing ~100 mmut zebrafish larvae. Plots represent mean ± SEM. Two-tailed Student’s t-test, *P < 0.05, **P < 0.01 and ^#P < 0.0001 relative to mmut^+/+ or HP-mmut^del11/del11 in a–f. One-way ANOVA followed by Bonferroni’s post hoc test, ***P < 0.001 relative to mmut^+/+ or 14-dpf-HP-fed mmut^+/+ or to 14-dpf-HP-fed mmut^del11/del11 zebrafish larvae in g. Scale bars are 5 μm in b and c, and 100 μm in e. NS non-significant. Source data are provided as a Source Data file.