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. 2020 Feb 20;10:3077. doi: 10.1038/s41598-020-59536-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The GGDEF domain, but not its activity, is needed for DgcP localization at the cell poles. msfGFP was fusioned to wild type DgcP (1–670), to a DgcP where the conserved GGEEF motif was mutated to GGAAF and to a truncated version without the GGDEF domain (1–560) and the fusions were produced from a plasmid in PA14. Only the wild-type and GGAAF fusions localize at the cell poles. White bars represent 4 μm.