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. 2020 Feb 20;10:3091. doi: 10.1038/s41598-020-59495-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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JHSB3 titer in the hemolymph of 4^th instar of R. prolixus. Nymphs were blood-fed and hemolymph was collected at different days after blood feeding until molting to 5th instar (10 days later). Day 0 represents insects just before feeding. Each data point represents the mean ± SEM of at least four independent replicates of hemolymph of groups of 15 nymphs each.