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. 2020 Feb 20;10:3091. doi: 10.1038/s41598-020-59495-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Developmental changes in the expression of JH biosynthetic enzyme mRNAs in the CA. Expression of JH biosynthetic enzymes mRNAs in CA of 4^th instar of R. prolixus. Nymphs were blood-fed and CAs were dissected at different days after blood feeding until molting to 5^th instar (10 days later). Enzyme mRNA bars represent the number of transcripts detected by RT-qPCR, and normalized using the expression of the rpL32 gene. Each RT-PCR data point is average of three independent biological replicates of 15 CA complexes. Enzyme abbreviations are as in Fig. 3.