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. 2020 Feb 20;11:978. doi: 10.1038/s41467-020-14739-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a PAGE analysis of stepwise assembly of DNA nanochannel. The bands of DNA nanochannel (lane 6) and assemblies of one to five different component strands (Lanes 1–5, respectively) are shown with high yield. L: 20 bp ladder. b Five percent PAGE analysis of ATP response of chol-DNGK. Lane 1: DNA nanochannel; lane 2: o-DNGK; lane 3: c-DNGK; lane 4: c-DNGK with addition of 3 mM ATP; Lane 5: chol-o-DNGK; Lane 6: chol-c-DNGK; Lane 7: chol-c-DNGK with addition of 3 mM ATP; Lane 8: blocker version DNGK with a blocker. Lane L: 20 bp DNA ladder. c Dynamic light scattering (DLS) verified the size changes between nanogatekeeper and chol-DNGK. Error bars show the standard deviation of three independent experiments. d Comparison of the small-angle X-ray scattering (SAXS) profile from o-DNGK (blue) with scattering profiles of chol-o-DNGK (green) and chol-c-DNGK (red), showing the changes between each group. e Fluorescence intensity shows the effect of adding various concentrations of ATP, which causes chol-c-DNGK switch to chol-o-DNGK, and the released locker (fluorophore labeled) enables the “OFF” to “ON” signal readout. f Response of c-DNGK to different analogs. The rate of fluorescence enhancement was measured in the presence of 3 mM ATP, guanosine-5’-triphosphate (GTP), cytidine triphosphate (CTP), uridine-5’-triphosphate (UTP) and a scrambled lid (SL) on nanogatekeeper with ATP, respectively. λ_ex = 492 nm, bandpass = 5 nm. Error bars show the standard deviation of three independent experiments. Source data are provided in the Source Data file.