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. 2020 Feb 20;10:3066. doi: 10.1038/s41598-020-59652-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antibacterial treatment of bacteria suspended in LB broth. Individual wells of a 24-well plate containing 1 × 10⁵ CFU/mL of S.aureus and E. coli suspended in 400 μL of LB broth were exposed to LTP for 3 minutes each. Samples were taken at 0, 1, 2, 3, and 4 hours and plated to determine CFU. Statistical significance was determined by one-way ANOVA with Tukey’s multiple-comparison test for treated samples and paired t-test for untreated sample. Significance is shown via grouping: a = significantly different from groups marked with b or c; b = significantly different from groups marked by a or c; c = significantly different from groups marked with a or b, p ≤ 0.05, N = 6.