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. 2020 Feb 20;10:3054. doi: 10.1038/s41598-020-59888-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression and Western blot. (a) Change in the ZO-1 gene expression determined from 3 independent experiments (n = 3). *p < 0.05 when compared to the control. (b) Western blot showing recovery of the ZO-1 protein level in response to P188 treatment is consistent with the gene expression measurements.