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. 2020 Feb 20;10:3119. doi: 10.1038/s41598-020-60098-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OSN isolation. (a) OSN nuclei are genetically tagged with a eGFP-Msp300^KASH that localizes to the nuclear membrane. (b) Cartoon showing a third-instar Drosophila larva with its two pairs of OSN bundles in green to depict expression of the eGFP-Msp300^KASH construct (c) GFP-tagged nuclei (green circles) are separated and purified from other nuclei (grey circles) using a magnetic pull-down method (see materials and methods). (d) Normalized gene expression levels of OSN specific genes (Orco, Or94b, eGFP) and more commonly expressed neural genes (syt1, Act42a) are evaluated after RNA purification from pre-isolation and post-isolation OSN-nuclei samples. Mean ± SEM. ***p < 0.0001.