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. 2020 Feb 20;10:3018. doi: 10.1038/s41598-020-59597-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression profile of AsWRKYs in MeJA treated calli. A. sinensis calli were transferred to MS containing 100 μM MeJA and sample at appointed times (0 h, 0.5 h, 2 h, 4 h, 6 h, 12 h, 24 h). Expression levels of all detected genes were assayed using real-time PCR analysis and AsGADPH as the internal control. Each value is the mean ± SE of 3 independent biological replicates. Asterisks indicate significant differences between each treatment point and controls (0-h time point) (*P < 0.05, **P < 0.01, Student’s t-tests).