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. 2020 Feb 20;10:3078. doi: 10.1038/s41598-020-59743-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NIPA2 expression and mitophagy in primary osteoblasts. (A) Immunofluorescence showing ALP expression in primary osteoblasts of each group. (B) ALP staining images of primary osteoblasts in WT and db/db groups. (C) Immunofluorescence of NIPA2 in primary osteoblasts of WT and db/db groups (values are presented as the mean ± SD; *P < 0.05 vs. WT, n = 30 cells from three independent experiments). (D) Detection of magnesium fluorescence in primary osteoblasts of WT and db/db groups (values are presented as the mean ± SD; *P < 0.05 vs. WT, n = 30 cells from three independent experiments). (E) TEM shows the number of mitophagosomes in primary osteoblasts of WT and db/db groups (scale bars, 1 μm and 2 μm. n = 3 per group. Values are presented as the mean ± SD *P < 0.05 vs. WT).