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. 2020 Feb 20;10:3078. doi: 10.1038/s41598-020-59743-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of NIPA2 on mitophagy. (A) Western blot showing the NIPA2, P62, mitochondrial PINK1, Parkin and LC3 protein levels in hFOB1.19 cells after transfection and HG treatment for 72 h. (B) Detection of Mg²⁺ fluorescence after successful NIPA2 transfection in HG at 72 h. (C) Flow cytometry showing the MMP of transfected hFOB1.19 cells after HG treatment for 72 h. Values are presented as the mean ± SD of three independent experiments. *P < 0.05 vs. HG. (D) Quantitative analysis of transfected hFOB1.19 cells treated with HG for 72 h that demonstrate colocalization of LC3 dots with mitochondria per cell (*P < 0.05, n = 9 cells from three independent experiments; Scale bar, 20 μm, values are presented as the mean ± SD). Data are expressed as the fold induction relative to HG.