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Primer sequences used in real- time PCR experiments.
| Gene | Primer sequence 5′-3′ |
|---|---|
| NIPA2 | F: CAGTTATGGTCATTCATGCTCCAA |
| R: TTAATATCAAGGCCACAATGACCAC | |
| Collagen-I | F: AGAGCTTCGGCAGCAGGA |
| R: CTTATAGCAGTTCTGCCTGC | |
| ALP | F: AACATCAGGGACATTGACGTG |
| R: GTATCTCGGTTTGAAGCTCTTCC | |
| OPG | F: GCGCTCGTGTTTCTGGACA |
| R: AGTATAGACACTCGTCACTGGTG | |
| OCN | F: CACTCCTCGCCCTATTGGC |
| R: CCCTCCTGCTTGGACACAAAG | |
| Runx-2 | F: CCT TCCAGACCAGCAGCAG |
| R: TCCGTCAGCGTCAACACCA | |
| β-actin | F: GACAGGATGCAGAAGGAGATTACT |
| R: TGATCCACATCTGCTG GAAGGT |
