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. 2020 Feb 20;10:3087. doi: 10.1038/s41598-020-59926-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of coiled-coil peptide-mediated membrane fusion between GUVs and LUVs. GUVs are functionalized with lipopeptide CP_nK₄ (red) and LUVs with CP_nE₄ (blue). Lipid-mixing is shown on the left and content-mixing on the right of the upper panel. (A) Spontaneous incorporation of the lipidated peptide into the lipid membrane via the cholesterol anchor results in the functionalization of GUVs with CP_nK₄. (B) Addition of CP_nE₄-functionalized LUVs to CP_nK₄-functionalized GUVs leads to the formation of a coiled-coil complex which triggers fusion. (C) Transfer of the fluorescent lipids and mixing of the inner aqueous contents of the GUV after fusion with LUVs represents lipid- and content-mixing. (D) The structures of the CP_nK₄ and CP_nE₄ lipopeptides employed in this study.