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. 2020 Feb 20;10:3061. doi: 10.1038/s41598-020-59898-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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H-ferritin-deficient macrophages retain less exogenously added iron. Iron was quantified in Fth1^+/+ (black) and Fth1^−/− (grey) BMDM treated with 10 μM of FAC or 100 μM of hemin. (a) Iron quantification by TEM-EDS at 30 minutes, 1 or 12 hours, post-treatments. The results represent the mean + SD of 6 to 24 cells and are expressed as the percentage of iron mass inside a single cell (two-way ANOVA with Tukey multiple comparisons test; *p < 0.05). (b) Iron quantification by atomic absorption at 24 h post-treatments. The results represent the mean + SD of 4 independent cultures per condition and are expressed as the ratio between the concentration of iron and the concentration of phosphorus in each sample (two-way ANOVA with Tukey multiple comparisons test; *p < 0.05).