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. 2020 Feb 20;10:3069. doi: 10.1038/s41598-020-59430-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of skeletal muscle-specific smPit1^−/−; smPit2^−/− double knockout mice. (A) Mating scheme used to target ablation of Pit1 and Pit2 in postnatal skeletal muscle with the human skeletal actin (HSA) promoter Cre. (B) Genotyping PCR for Pit1^fl, HSA-Cre, and Pit1^wt. (C) Genotyping PCR for the recombinant Pit1^delta3,4 and Pit2^delta4 in various tissues of a smPit1^−/−; smPit2^−/− mouse at P10. For raw images see supplement. (D) Semi-quantitative qenomic PCR showed 20% reduced expression of the recombinant Pit1^delta3,4 in heart when compared to quadriceps. For raw images see supplement. (E) Semi-quantitative RT-PCR showed reduced expression of Pit1 and Pit2 in quadriceps muscle of smPit1^−/− and smPit2^−/− mice at P80, respectively. Means ± SEM, n = 5–8, ^***p = 0.0002, ^**p = 0.002, ^*p = 0.03 vs. WT.